EconoSpin™
EconoSpin™ silica membrane and spin column substitute for DNA and RNA purification. Available to customers in Japan.
EconoSpinTM
Silica Membrane and Spin Columns for DNA and RNA Purification
Product Details
EconoSpin™ Silica Membrane & Spin Column for DNA
- Buffer and protocol of another company’s nucleic acid purification kit can be used
- Possible to use the remaining buffer without waste
- Economical
- Choose between with / without lid
- Create your own solution by buffered recipe
Main usage:
- PCR product purification
- Plasmid purification
- Agarose gel DNA extraction
- Buffer exchange of restriction enzyme reaction
- Probe label cleanup
Performance comparison with Other Products
Example: Purification yield of plasmid from 1 ml culture broth (μg)
Results: The same plasma yield is confirmed as when using a column manufactured by Company A
Example: Purity – Protein contamination
Results: A value serving as an indicator of protein contamination A260 / 280 > 1.7
(the value decreases due to protein contamination)
EconoSpin™ Silica Membrane & Spin Column for RNA
EconoSpin™ Silica Membrane & Spin Column is RNase-free spin column that is popular for nucleic acid purification and safe for RNA purification.
RNA purification example include cultured cells, bacteria, plants, and yeast.
EconoSpin™ Empty Column
- Filled the desired carrier
- Used for preparing affinity columns
- Used for preparing ion exchange columns
- Application example: Fill the Ni agarose carrier and purify the His tagged protein
Frequently Asked Questions
Which kit of which maker corresponds to which EconoSpin™ kit?
Although the main purpose of EconoSpin™ is a spin column substitute included in a commercially available nucleic acid purification kit, it is difficult to conduct a conformity test for each of the various types of kits that are currently offered.
In order to test whether EconoSpin™ corresponds to various kits, Ajinomoto Bio-Pharma Services can prepare a free sample. If you would like a sample, contact the Aji Bio-Pharma team.
Why is the DNA yield low (cannot be obtained) with EconoSpin™?
EconoSpin™ may be lower in yield than dedicated columns of each kit’s genuine product due to its character as a substitute. In addition, there is a possibility not to adapt depending on the purification method of each kit and the type of buffer.
What is the length of DNA that can be recovered by EconoSpin™?
The DNA chain length that can be collected by EconoSpin™ with high probability is 100 to 10000 bp.
What is the elution volume of EconoSpin™?
Depending on the experiment, the elution volume of EconoSpin™ is approximately 10 to 50 μL.
What is the storage temperature of EconoSpin™?
EconoSpin™ can be stored at room temperature.
Is there a buffer recipe for EconoSpin™ for RNA?
A buffer recipe is not provided for the EconoSpin™ RNA column.
What is the difference between EconoSpin™ with lid and without lid?
EconoSpin™ with lid makes it possible to prevent the liquid from popping out when centrifuging. EconoSpin™ without lid allows work to be done efficiently because there is no need to open and close the lid.
Since the amount of silica membrane is the same in both cases, there is no difference in the amount of DNA recovered. For RNA, EconoSpin™ is only offered with lid.
