miRNA Products
We offer both miRNA mimic and inhibitor oligonucleotides to support a range of research applications.
miRNA Mimic
miRNA mimics contain non-natural or artificial double-stranded miRNA-like RNA fragments, primarily used to study gene function by targeting mRNA through miRNA-like activity in mammalian cells.
miRNA mimics are manufactured and sold for research and development purposes based on results obtained through collaborative research with Cytopathfinder Co., Ltd.
Product Details
Overview
miRNA mimic oligonucleotides are double-stranded RNA oligonucleotides with a carefully designed and chemically modified strand, which can be used for targeted gene suppression, antisense, and cell reprogramming.
Our miRNA oligonucleotides consist of a guide strand with natural RNA and a passenger strand with chemical modifications. The guide strand shows strong RNAi activity. The passenger strand shows no RNAi activity.
miRNA Inhibitor
miRNA inhibitors are chemically modified, single-stranded oligos designed to bind to and inhibit microRNA (miRNA) molecules. Our miRNA inhibitor oligonucleotides, S-TuD™, are available to customers in Japan.
S-TuD™ is manufactured in Japan for R&D purposes based on licensing of the invention (Patent No. 496343) agreements with the University of Tokyo.
Product Details
Overview
miRNA Inhibitor oligos are double-stranded RNA mainly composed of 2′-O-Methyl RNA. The Micro RNA Binding Site (MBS) specifically binds to RISC-complexed mature miRNA and strongly inhibits miRNA activity even at low concentrations.
We offer an innovative miRNA inhibitor, S-TuD™, which shows significantly higher inhibitory activity against miRNA than existing miRNA inhibitors.
When the inhibitory activity against miRNA was confirmed, S-TuD™ showed inhibitory activity at significantly lower concentrations than existing miRNA inhibitors.
Our miRNA inhibitor, S-TuD™, was transfected into HCT-116 cells by transfection method and the inhibitory activity against each miRNA was confirmed by Dual Luciferase Assay. S-TuD™ shows inhibitory activity at significantly lower concentrations than existing miRNA inhibitors. Data was provided by Professor Iba of the University of Tokyo Institute of Medical Science.
Reference: Haraguchi, T., Nakano, H., Tagawa, T., Ohki, T., Ueno, Y., and Iba, H. 2012. A potent
2′- O- methylated RNA-based microRNA inhibitor with unique secondary structures. Nucleic Acids Res., 40: modified from e 58
