siRNA Liquid-Phase Synthesis
Our AJIPHASE™ technology utilizes enzyme-mediated ligation of multiple RNA fragments to produce target siRNA compounds with higher yield and purity compared to conventional solid-phase synthesis. This method enables the production of GMP-grade products in quantities ranging from several kilograms to several hundred kilograms.
Production by liquid-phase synthesis technology, AJIPHASE™
Product Details
High purity
The short length of the synthesized fragments allows for high-purity production. Additionally, in the enzyme ligation process, impurities (such as N±1 mer) can be effectively removed, allowing the omission of the purification step for synthetic fragments. Furthermore, there is minimal residual complementary strand after ligation, resulting in the acquisition of the final product with high quality.
High productivity
The ability to obtain high-quality intermediates enhances purification efficiency compared to general production methods. This technology also ensures high reproducibility and productivity, even in large-scale production.
Reduction of environmental impact
By achieving high productivity, the amount of raw materials used can be reduced, thereby lowering the environmental impact throughout the oligonucleotide production supply chain.
This technology offers a sustainable solution that enhances quality and efficiency in pharmaceutical manufacturing while being environmentally conscious.
WO2020171092 Method For Producing Modified Oligonucleotide Including Complementary Sequence
